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Oxford Instruments
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Proteintech
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Cell Signaling Technology Inc
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Proteintech
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Proteintech
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Proteintech
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Cell Signaling Technology Inc
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Proteintech
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Journal: Molecular Reproduction and Development
Article Title: High‐Resolution Imaging Reveals Mitochondrial Protein Imbalance in Sperm of Oligoasthenospermic Men
doi: 10.1002/mrd.70110
Figure Lengend Snippet: The origin of the sperm, control, or oligoasthenospermic influence the mitochondrial area. (A) Representative images obtained by immunofluorescence staining of spermatozoa with typical morphology. (B) An example of atypical morphology of spermatozoa with defects in the head and middle piece. Mitochondria were immunostained by anti‐TOM20 antibodies (orange), scale bar = 1 µm. (C) Representative images of the probes MitoTracker Green, the blue dots delimited the head of the spermatozoon, scale bar = 1 µm. (D) Mitochondrial area assessed by TOM20. Results are presented with a box‐and‐whiskers plot, each box represents the interquartile range (25th–75th percentile), with the horizontal line indicating the median. Whiskers extend to the maximum from the minimum. Total control (Ct) n = 760 spermatozoa; Total oligoasthenospermic (OA) n = 612; Typical Ct n = 179; Atypical Ct n = 581; Typical OA n = 118; Atypical OA n = 494. The p‐ value of the two‐sided permutation t‐test is represented as * p < 0.05; ** p ≤ 0.01; *** p ≤ 0.001.
Article Snippet: The resulting surface accurately reflected the size and shape of the
Techniques: Control, Immunofluorescence, Staining